Table 2 Oligonucleotides
Primer name | Sequence (5′–3′) | Use |
|---|---|---|
DyhaM-FW | ATGTTTGATTCGACTTTAAATCCGTTATGGCAGCGTTACATCCTCGCCGTTGAAGCCTGCTTTTTTATACTAAGTTGGCA | Construction of ΔyhaM |
DyhaM-RV | TTATCTGGCCTTGCTCGCCATAATCTCGATAATCTGCCGATCCGTTTGCTCGCTCAAGTTAGTATAAAAAAGCTGAACGA | Construction of ΔyhaM |
ECOydeD-F | CGCGGATCCAATGGTCATAAATGGCAGCGTAGCGC | Cloning of ydeD |
ECOydeD-R | CGCGGATCCGCAGGGCGTTGCGGAACAAAC | Cloning of ydeD |
ECOyhaM-F | CGCGGATCCAAGATGCCTGCCGAGAAGATTAACG | Cloning of yhaM |
ECOyhaM-R | CGCGGATCCGAGCGAGCTGGAAGCTATCG | Cloning of yhaM |
yhaM(Ec)RT-F | CTCGATTCCGCGAAGCTAAA | Real-time PCR: detection of yhaM |
yhaM(Ec)RT-R | CCCCCACTTACCGCTCAA | Real-time PCR: detection of yhaM |
yhaO(Ec)RT-F | GCCATTATTACGCTGCCGTTT | Real-time PCR: detection of yhaO |
yhaO(Ec)RT-R | CCATCGGACTTAACGTCTGGAT | Real-time PCR: detection of yhaO |
metC(Ec)RT-F | AAGCCGCCACCAAATATCTG | Real-time PCR: detection of metC |
metC(Ec)RT-R | ACACGGCAGTGCCAATCA | Real-time PCR: detection of metC |