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DyhaM-FW
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ATGTTTGATTCGACTTTAAATCCGTTATGGCAGCGTTACATCCTCGCCGTTGAAGCCTGCTTTTTTATACTAAGTTGGCA
|
Construction of ΔyhaM
|
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DyhaM-RV
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TTATCTGGCCTTGCTCGCCATAATCTCGATAATCTGCCGATCCGTTTGCTCGCTCAAGTTAGTATAAAAAAGCTGAACGA
|
Construction of ΔyhaM
|
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ECOydeD-F
|
CGCGGATCCAATGGTCATAAATGGCAGCGTAGCGC
|
Cloning of ydeD
|
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ECOydeD-R
|
CGCGGATCCGCAGGGCGTTGCGGAACAAAC
|
Cloning of ydeD
|
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ECOyhaM-F
|
CGCGGATCCAAGATGCCTGCCGAGAAGATTAACG
|
Cloning of yhaM
|
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ECOyhaM-R
|
CGCGGATCCGAGCGAGCTGGAAGCTATCG
|
Cloning of yhaM
|
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yhaM(Ec)RT-F
|
CTCGATTCCGCGAAGCTAAA
|
Real-time PCR: detection of yhaM
|
|
yhaM(Ec)RT-R
|
CCCCCACTTACCGCTCAA
|
Real-time PCR: detection of yhaM
|
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yhaO(Ec)RT-F
|
GCCATTATTACGCTGCCGTTT
|
Real-time PCR: detection of yhaO
|
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yhaO(Ec)RT-R
|
CCATCGGACTTAACGTCTGGAT
|
Real-time PCR: detection of yhaO
|
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metC(Ec)RT-F
|
AAGCCGCCACCAAATATCTG
|
Real-time PCR: detection of metC
|
|
metC(Ec)RT-R
|
ACACGGCAGTGCCAATCA
|
Real-time PCR: detection of metC
|
