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Table 2 Oligonucleotides
| Primer name | Sequence (5′–3′) | Use |
|---|---|---|
| DyhaM-FW | ATGTTTGATTCGACTTTAAATCCGTTATGGCAGCGTTACATCCTCGCCGTTGAAGCCTGCTTTTTTATACTAAGTTGGCA | Construction of ΔyhaM |
| DyhaM-RV | TTATCTGGCCTTGCTCGCCATAATCTCGATAATCTGCCGATCCGTTTGCTCGCTCAAGTTAGTATAAAAAAGCTGAACGA | Construction of ΔyhaM |
| ECOydeD-F | CGCGGATCCAATGGTCATAAATGGCAGCGTAGCGC | Cloning of ydeD |
| ECOydeD-R | CGCGGATCCGCAGGGCGTTGCGGAACAAAC | Cloning of ydeD |
| ECOyhaM-F | CGCGGATCCAAGATGCCTGCCGAGAAGATTAACG | Cloning of yhaM |
| ECOyhaM-R | CGCGGATCCGAGCGAGCTGGAAGCTATCG | Cloning of yhaM |
| yhaM(Ec)RT-F | CTCGATTCCGCGAAGCTAAA | Real-time PCR: detection of yhaM |
| yhaM(Ec)RT-R | CCCCCACTTACCGCTCAA | Real-time PCR: detection of yhaM |
| yhaO(Ec)RT-F | GCCATTATTACGCTGCCGTTT | Real-time PCR: detection of yhaO |
| yhaO(Ec)RT-R | CCATCGGACTTAACGTCTGGAT | Real-time PCR: detection of yhaO |
| metC(Ec)RT-F | AAGCCGCCACCAAATATCTG | Real-time PCR: detection of metC |
| metC(Ec)RT-R | ACACGGCAGTGCCAATCA | Real-time PCR: detection of metC |
