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Table 1 Strains and plasmids

From: Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli

Strains and plasmids Descriptiona Source or reference
Strains
 MG1655 Wild-type E. coli MG1655 (ATCC 47076) ATCC
 MG1655ΔyhaM MG1655ΔyhaM This study
Plasmids
 pSTV29 Cloning vector, p15a ori, Cmr Takara
 pSTV-ydeD5 pSTV29; ydeD (E. coli) gene containing upstream 300 bp and 200 bp downstream This study
 pSTV-yhaM7 pSTV29; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream This study
 pACYC177 Cloning vector, p15a ori, Kmr Nippon Gene
 pACYC-yhaM1 pACYC177; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream This study
 pMIV-cysE5 cysE5 (E. coli) under the control of the ompC promoter, p15a ori, Cmr (Nonaka and Takumi 2011)
  1. ATCC American Type Culture Collection
  2. aCmr, Kmr, resistance to chloramphenicol and kanamycin