Strains and plasmids | Descriptiona | Source or reference |
---|---|---|
Strains | ||
 MG1655 | Wild-type E. coli MG1655 (ATCC 47076) | ATCC |
 MG1655ΔyhaM | MG1655ΔyhaM | This study |
Plasmids | ||
 pSTV29 | Cloning vector, p15a ori, Cmr | Takara |
 pSTV-ydeD5 | pSTV29; ydeD (E. coli) gene containing upstream 300 bp and 200 bp downstream | This study |
 pSTV-yhaM7 | pSTV29; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream | This study |
 pACYC177 | Cloning vector, p15a ori, Kmr | Nippon Gene |
 pACYC-yhaM1 | pACYC177; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream | This study |
 pMIV-cysE5 | cysE5 (E. coli) under the control of the ompC promoter, p15a ori, Cmr | (Nonaka and Takumi 2011) |