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Table 1 Strains and plasmids

From: Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli

Strains and plasmids

Descriptiona

Source or reference

Strains

 MG1655

Wild-type E. coli MG1655 (ATCC 47076)

ATCC

 MG1655ΔyhaM

MG1655ΔyhaM

This study

Plasmids

 pSTV29

Cloning vector, p15a ori, Cmr

Takara

 pSTV-ydeD5

pSTV29; ydeD (E. coli) gene containing upstream 300 bp and 200 bp downstream

This study

 pSTV-yhaM7

pSTV29; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream

This study

 pACYC177

Cloning vector, p15a ori, Kmr

Nippon Gene

 pACYC-yhaM1

pACYC177; yhaM (E. coli) gene containing upstream 300 and 200 bp downstream

This study

 pMIV-cysE5

cysE5 (E. coli) under the control of the ompC promoter, p15a ori, Cmr

(Nonaka and Takumi 2011)

  1. ATCC American Type Culture Collection
  2. aCmr, Kmr, resistance to chloramphenicol and kanamycin