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Table 2 Bacterial laccases, laccase-like phenol oxidases and multi-copper oxidases produced by heterologous expression in E. coli

From: Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications

Enzyme

Origin

Yield

Comments

Reference

PpoA

Marinomonas mediterranea

NR

 

Sanchez-Amat et al. (2001)

Cot A

Bacillus subtilis

NR

10% of total recombinant CotA expressed by E. coli was purified successfully

Martins et al. (2002)

CotA

B. subtilis

20 mg L−1 (aerobic)

23 mg L−1 (anaerobic)

 

Durao et al. (2006, 2008a, b); Pereira et al. (2009)

EpoA

Streptomyces griseus

NR

 

Endo et al. (2003)

STSL

Streptomyces lavendulae

10 mg L−1

 

Suzuki et al. (2003)

Lbh1

Bacillus halodurans

NR

 

Ruijssenaars and Hartmans (2004)

SLAC

Streptomyces coelicolor

NR

 

Machczynski et al. (2004)

Tth laccase

Thermus thermophilus

10 mg L−1

 

Miyazaki (2005)

McoA

Aquifex aeolicus

NR

Expressed as insoluble protein and recovered by unfolding and refolding

Fernandes et al. (2007)

CotA

Bacillus licheniformis

10 mg L−1

 

Koschorreck et al. (2008)

SilA

Streptomyces ipomea

NR

4.8-fold purification of the protein corresponding to a final yield of 85.6%

Molina-Guijarro et al. (2009)

LCMOs

Bacillus coagulans

Bacillus clausii

Bacillus pumilus

Streptomyces pristinaespiralis

Gramella forsetii

Marivirga tractuosa

Spirosoma linguale

NR

 

Ihssen et al. (2015)

SCLAC

SLLAC

SVLAC

AMLAC

S. coelicolor

Streptomyces lividans

Streptomyces viridosporus

Amycolatopsis sp.

15–20 mg L−1

 

Majumdar et al. (2014)

Cot A

B. clausii

NR

 

Brander et al. (2014)

Ssl1

Streptomyces sviceus

40–50 mg L−1

 

Gunne and Urlacher (2012)

BALL

B. licheniformis

20 mg L−1

 

Tonin et al. (2016)

MmPPO

Marinomonas mediterranea

NR

Recovered from membrane fraction and partially purified to 34 U L−1

Tonin et al. (2016)

  1. The enzyme yield is the amount of enzyme recovered after purification
  2. NR not reported