Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

AMB Express

Table 1 Comparison of DHA content, DHA productivity, lipid and DHA yield of C. cohnii under different N-sources

N-source	Growth stage	C_DHA (g/L)	P_DHA (mg/L day)	Y ^a_L (mg/g GLC)	Y ^a_DHA (mg/g GLC)
(NH₄)₂SO₄	Growth	0.05 ± 0.0	22.4 ± 4.9	35.5 ± 2.2	3.7 ± 0.7
	Lipid accumulation	0.35 ± 0.1	70.9 ± 8.9	65.6 ± 1.4	16.9 ± 2.5
	Lipid turnover	0.42 ± 0.1	60.3 ± 7.1	71.3 ± 5.3	19.7 ± 1.5
NH₄HCO₃	Growth	0.05 ± 0.0	25.8 ± 1.6	28.9 ± 4.2	3.9 ± 1.2
	Lipid accumulation	0.39 ± 0.08	77.3 ± 3.9	74.4 ± 1.1	17.6 ± 0.4
	Lipid turnover	0.43 ± 0.05	60.7 ± 4.4	66.8 ± 3.5	19.1 ± 1.5
(NH₂)₂CO	Growth	0.06 ± 0.0	27.5 ± 3.1	34.3 ± 6.9	4.2 ± 1.5
	Lipid accumulation	0.46 ± 0.05	91.1 ± 3.2	75.1 ± 2.2	20.7 ± 0.6
	Lipid turnover	0.46 ± 0.11	65.3 ± 4.2	73.4 ± 3.4	20.6 ± 0.9
NaNO₃	Growth	0.13 ± 0.02	63.4 ± 1.6	43.1 ± 3.5	7.4 ± 1.4
	Lipid accumulation	0.97 ± 0.12	193.8 ± 2.8	130.3 ± 4.5	44.1 ± 3.6
	Lipid turnover	0.99 ± 0.05	141.1 ± 5.7	127.1 ± 9.6	44.1 ± 2.4

All experiments were performed in triplicate. The data presented here is mean ± SD
C _DHA DHA content, P _DHA DHA productivity (g/L day), Y _L lipid yield (mg/g glucose), Y _DHA DHA yield (g/g glucose)
^a Yield was calculated on basis of glucose utilized