Fig. 1
From: Degradation and inactivation of Shiga toxins by nitrogen gas plasma
Immunochromatography of Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2) after nitrogen gas plasma treatment. A coverslip containing dried spots from 20 μl aliquots of a 1 μg/ml solution of Shiga toxins (Stx) including Stx1 (a) and Stx2 (b) was treated with nitrogen gas plasma using a bi-polar and low-pressure plasma-triple effects sterilization (BLP-TES) device (1.5 kpps) for 0, 5, 15, or 30 min. The recovered Stx1 and Stx2 samples were analyzed by immunochromatography using an NH immunochromato VT1/2 kit (NH Foods Ltd., Osaka, Japan). Test lines for Stx1 and Stx2, and reference lines for the internal control are indicated by arrows