Nitrate decreases methane production also by increasing methane oxidation through stimulating NC10 population in ruminal culture

AMB Express

Table 1 The specific primers for PCR and qPCR

Target organisms	Primer	Primer sequence (5′–3′)	References
Total bacteria	Forward^b	ACTCCTACGGGAGGCAGCAGT	Schwiertz et al. (2010)
Total bacteria	Reverse^b	ATTACCGCGGCTGCTGGC	Schwiertz et al. (2010)
Methanogens (mcrA)	Forward^b	AACGCTAGCTACAGGCTT	Denman et al. (2007)
Methanogens (mcrA)	Reverse^b	CCAATGTGGGGGACCTTC	Denman et al. (2007)
NC10	qP1mF^b	GGGCTTGACATCCCACGAACCTR	He et al. (2016)
NC10	qP1R^b	GCGTCAAGCTGGAGGAAGGCG	He et al. (2016)
Anammox	Pla46F^a	GGATTAGGCATGCAAGTC	Amano et al. (2007)
	AMX1480R^a	TACGACTTAGTCCTCCTCAC	Amano et al. (2007)
	amoA-1F^b	GGGGTTTCTACTGGTGGT	Li et al. (2009)
	amoA-2R^b	CCCCTCKGSAAAGCCTTCTTC	Li et al. (2009)
ANME-2d	DP142F^a	TAATACYGGATAGATCAAAG	Ding et al. (2015)
	DP779R^a	GCACCGCACCTGACACCT
	DP397F^b	TGGCTGTCCAGCTRTYC
	DP569R^c	GRACGCCTGACGATTRAG