Fig. 4
From: Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris
Changes in gene expression for elements of the UPR for VRC01 expressing strains. RT-qPCR was used to determine gene expression for KAR2, PDI¸ total HAC1 and spliced HAC1. ACT1 was used as the housekeeping gene. Fold change was calculated using the Pfaffl calculation, as a comparison to Δku70 0 h. Error bars are a representation of Gaussian error propagation from three technical replicates of three biological replicates. Red asterisks indicate a significant difference compared to Δku70 at 0 h, where p ≤ 0.05. a Spliced HAC1; b KAR2; c PDI1; d Total HAC1