Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

AMB Express

Table 3 Summary of the rhamnolipids extracted from cultures of P. aeruginosa ST5, as detected with high resolution mass spectrometry (<10 ppm)

Rhamnolipid group (Abbr)	UPLC Rt (min)^a	Proposed structures of rhamnolipids	Mono-isotopic Exp/Theor M _r	Protonated species Exp/Theor m/z	Sodiated species Exp/Theor m/z	Sodiated dimeric species Exp/Theor m/z
mRL1	7.46	Rha–C₈–C₁₀	476.3047	477.3089	499.2896	975.5889
mRL1	7.46	Rha–C₁₀–C₈	476.2985	477.3063	499.2883	975.5868
dRL1	6.6	Rha–Rha–C₈–C₁₀ Rha–Rha–C₁₀–C₈	622.3576	623.3654	645.3471	1267.7074
dRL1	6.5	Rha–Rha–C₈–C₁₀ Rha–Rha–C₁₀–C₈	622.3564	623.3642	645.3462	1267.7026
mRL2	9.03	Rha–C₁₀–C₁₀	504.3305	505.3383	527.3201	1031.6501
mRL2	9.03	Rha–C₁₀–C₁₀	504.3298	505.3376	527.3196	1031.6494
dRL2	7.69, 7.85, 8.07, 8.25, 8.42	Rha–Rha–C₁₀–C₁₀	650.3894	651.3972	673.3772	1323.7701
dRL2	7.69, 7.85, 8.07, 8.25, 8.42	Rha–Rha–C₁₀–C₁₀	650.3877	651.3955	673.3775	1323.7652
mRL3	10.56	Rha–C₁₂–C₁₀	532.3640	533.3700	555.3546	1087.7201
mRL3	10.56	Rha–C₁₀–C₁₂	532.3611	533.3689	555.3509	1087.7120
dRL3	9.6	Rha–Rha–C₁₂–C₁₀	678.4177	679.4285	701.4114	1379.8352
dRL3	9.7	Rha–Rha–C₁₀–C₁₂	678.4190	679.4268	701.4088	1379.8278

The proposed chemical structures, theoretical (Theor) and experimental (Exp) M _r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
^aUPLC Retention time of main peaks corresponding to the group’s m/z value