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Table 3 Summary of the rhamnolipids extracted from cultures of P. aeruginosa ST5, as detected with high resolution mass spectrometry (<10 ppm)

From: Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

Rhamnolipid group (Abbr) UPLC Rt (min)a Proposed structures of rhamnolipids Mono-isotopic
Exp/Theor
M r
Protonated species
Exp/Theor
m/z
Sodiated species Exp/Theor
m/z
Sodiated dimeric species Exp/Theor
m/z
mRL1 7.46 Rha–C8–C10 476.3047 477.3089 499.2896 975.5889
Rha–C10–C8 476.2985 477.3063 499.2883 975.5868
dRL1 6.6 Rha–Rha–C8–C10 Rha–Rha–C10–C8 622.3576 623.3654 645.3471 1267.7074
6.5 622.3564 623.3642 645.3462 1267.7026
mRL2 9.03 Rha–C10–C10 504.3305 505.3383 527.3201 1031.6501
504.3298 505.3376 527.3196 1031.6494
dRL2 7.69, 7.85, 8.07, 8.25, 8.42 Rha–Rha–C10–C10 650.3894 651.3972 673.3772 1323.7701
650.3877 651.3955 673.3775 1323.7652
mRL3 10.56 Rha–C12–C10 532.3640 533.3700 555.3546 1087.7201
Rha–C10–C12 532.3611 533.3689 555.3509 1087.7120
dRL3 9.6 Rha–Rha–C12–C10 678.4177 679.4285 701.4114 1379.8352
9.7 Rha–Rha–C10–C12 678.4190 679.4268 701.4088 1379.8278
  1. The proposed chemical structures, theoretical (Theor) and experimental (Exp) M r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
  2. aUPLC Retention time of main peaks corresponding to the group’s m/z value