Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

Ndlovu, Thando; Rautenbach, Marina; Khan, Sehaam; Khan, Wesaal

doi:10.1186/s13568-017-0367-4

AMB Express

Table 1 Summary of the surfactins extracted from B. amyloliquefaciens ST34, as detected with high resolution mass spectrometry (<10 ppm)

From: Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

Surfactin group (Abbr)	R_t (min)^a	Characterised and proposed* peptide sequences in surfactin group	Mono-isotopic Exp/Theor M _r	Protonated species Exp/Theor m/z	Sodiated species Exp/Theorm/z
Surfactin 1 (Srf1)	10.7; 10.8; 11.5; 11.6	Cyclo[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Leu-d-Leu-l-Val-l-Asp-l-Leu-l -Val]	993.6376	994.6473	1016.6365
Surfactin 1 (Srf1)	10.7; 10.8; 11.5; 11.6	Cyclo[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Val]	993.6403	994.6481	1016.6259
Surfactin 2 (Srf2)	11.3; 11.4; 12.1; 12.2	Cyclo[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Val] Cyclo[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Val]	1007.6565	1008.6608	1030.6415
		Cyclo-[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] Cyclo[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Ile] Cyclo-[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] Cyclo-[(C ₁₃ H ₂₄ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Ile]	1007.6565	1008.6608	1030.6415
			1007.6552	1008.6596	1030.6416
Surfactin 3 (Srf3)	11.8; 11.9; 12.0	Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Val] Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Val]	1021.6693	1022.6773	1044.6582
		Cyclo[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] cyclo[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Ile] Cyclo-[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] Cyclo-[(C ₁₄ H ₂₆ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Ile]	1021.6693	1022.6773	1044.6582
			1021.6715	1022.6752	1044.6572
Surfactin 4 (Srf4)	12.4	Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Leu-D-Leu-l-Val-l-Asp-l-Leu-l -Ile] Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Leu] Cyclo[(C ₁₅ H ₂₈ O ₂)-l-Glu-l -Ile-D-Leu-l-Val-l-Asp-l-Leu-l -Ile*]	1035.6819	1036.6896	1058.6737
Surfactin 4 (Srf4)	12.4		1035.6881	1036.6909	1058.6729

The proposed chemical structures, theoretical (Theor) and experimental (Exp) M _r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
^aUPLC retention time of main peaks corresponding to the groups m/z values

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