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Table 3 Summary of the detected surfactin lipopeptides extracted from cultures of B. amyloliquefaciens ST34, as detected using high resolution mass spectrometry (<10 ppm)

From: Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant

Surfactin group (Abbr) UPLC Rt (min)a Characterised and proposed* peptide sequences in surfactin group Mono-isotopic Exp/Theor M r Protonated specie Exp/Theor m/z Sodiated specie Exp/Theor m/z
Surfactin 1 (Srf1) 10.6; 11.2 Cyclo[(C 13 H 24 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
Cyclo[(C 13 H 24 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
993.6376
993.6403
994.6472
994.6481
1016.6265
1016.6190
Surfactin 2 (Srf2) 11.0; 11.2; 11.9 Cyclo[(C 14 H 26 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
Cyclo[(C 14 H 26 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
1007.6521
1007.6552
1008.6604
1008.6596
1030.6350
1030.6328
Cyclo-[(C 13 H 24 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
Cyclo[(C 13 H 24 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
*Cyclo-[(C 13 H 24 O 2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
*Cyclo-[(C 13 H 24 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
Surfactin 3 (Srf3) 11.6; 11.7; 12.3 Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]
1021.6693
1021.6715
1022.6780
1022.6752
1044.6586
1044.6494
Cyclo[(C 14 H 26 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
Cyclo[(C 14 H 26 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
*Cyclo-[(C 14 H 26 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
*Cyclo-[(C 14 H 26 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
Surfactin 4 (Srf4) 12.1; 12.2 Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
*Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
Cyclo[(C 15 H 28 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
1035.6819
1035.6881
1036.6902
1036.6909
1058.6718
1058.6662
Surfactin 5 (Srf5) 12.6; 12.7 Cyclo[(C 16 H 30 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
*Cyclo[(C 16 H 30 O 2 )-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
*Cyclo[(C 16 H 30 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu]
*Cyclo[(C 16 H 30 O 2 )-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]
1049.6992
1049.7032
1050.7120
1050.7066
1072.6926
1072.6886
  1. Their proposed chemical structures, theoretical (Theor) and experimental (Exp) M r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
  2. a UPLC retention time of main peaks corresponding to the group’s m/z value