Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant

Ndlovu, Thando; Rautenbach, Marina; Vosloo, Johann Arnold; Khan, Sehaam; Khan, Wesaal

doi:10.1186/s13568-017-0363-8

AMB Express

Table 3 Summary of the detected surfactin lipopeptides extracted from cultures of B. amyloliquefaciens ST34, as detected using high resolution mass spectrometry (<10 ppm)

From: Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant

Surfactin group (Abbr)	UPLC Rt (min)^a	Characterised and proposed* peptide sequences in surfactin group	Mono-isotopic Exp/Theor M _r	Protonated specie Exp/Theor m/z	Sodiated specie Exp/Theor m/z
Surfactin 1 (Srf1)	10.6; 11.2	Cyclo[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] Cyclo[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]	993.6376 993.6403	994.6472 994.6481	1016.6265 1016.6190
Surfactin 2 (Srf2)	11.0; 11.2; 11.9	Cyclo[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] Cyclo[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]	1007.6521 1007.6552	1008.6604 1008.6596	1030.6350 1030.6328
Surfactin 2 (Srf2)	11.0; 11.2; 11.9	Cyclo-[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] Cyclo-[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo-[(C ₁₃ H ₂₄ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]	1007.6521 1007.6552	1008.6604 1008.6596	1030.6350 1030.6328
Surfactin 3 (Srf3)	11.6; 11.7; 12.3	Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val]	1021.6693 1021.6715	1022.6780 1022.6752	1044.6586 1044.6494
Surfactin 3 (Srf3)	11.6; 11.7; 12.3	Cyclo[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] Cyclo-[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo-[(C ₁₄ H ₂₆ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile]	1021.6693 1021.6715	1022.6780 1022.6752	1044.6586 1044.6494
Surfactin 4 (Srf4)	12.1; 12.2	Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₅ H ₂₈ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile*]	1035.6819 1035.6881	1036.6902 1036.6909	1058.6718 1058.6662
Surfactin 5 (Srf5)	12.6; 12.7	Cyclo[(C ₁₆ H ₃₀ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₆ H ₃₀ O ₂)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] Cyclo[(C ₁₆ H ₃₀ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] Cyclo[(C ₁₆ H ₃₀ O ₂)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile*]	1049.6992 1049.7032	1050.7120 1050.7066	1072.6926 1072.6886

Their proposed chemical structures, theoretical (Theor) and experimental (Exp) M _r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
^a UPLC retention time of main peaks corresponding to the group’s m/z value

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