Table 2 Primers used in this study
Primer name | Sequence (5′ → 3′) |
|---|---|
Sequence analysis and cloning primers | |
MmTKL1-forward1 | GTCGAGTTGCCTTTGGTG |
MmTKL1-reverse1 | AGAACGAACCAAATCCAG |
MmTKL1-met-NotI-forward | AAAGCGGCCGCATGCCACTCAAATCGTTTGA |
MmTKL1-stop-NotI-reverse | AAAGCGGCCGCTTACGCCAACATTTCTTTGA |
MmTKL2-met-forward1 | ATGGTTGGGTTGGGTTGGGGCTGCCATGCT |
MmTKL2-stop-reverse1 | TTAAAGCAATTGTTTAACCTTCTTTTGAAT |
MmTKL2-forward2 | TACACTCCTGTTGGGCTC |
MmTKL2-reverse2 | CAGCTGCATCAACGACAC |
T7 | TAATACGACTCACTATAGGG |
sp6 | ATTTAGGTGACACTATAGAA |
ScTKL1-met-forward | ATGACTCAATTCACTGACATTGATAAGCTA |
ScTKL1-stop-reverse | TTAGAAAGCTTTTTTCAAAGGAGAAATTAG |
Gene expression analysis primers | |
GAPDH-RT-forward | CAAGGGTGGTGCCAAGAAGGT |
GAPDH-RT-reverse | CCTTGGGGTCGTACGATTCG |
MmTKL1-RT-forward | ACCTGGAAGGATCGACAGG |
MmTKL1-RT-reverse | CGGTAAGTACCTTCGGGT |
MmTKL2-RT-forward | CCTACTCGTCTGTACTTCCC |
MmTKL2-RT-reverse | CCGAGTCAGATCCTTCCT |