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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Complementary function of two transketolase isoforms from Moniliella megachiliensis in relation to stress response

Fig. 3

Complementation of Saccharomyces cerevisiae tkl1 mutant by MmTKL1 and MmTKL2. This assay tested complementation of the aromatic amino acid (AAA) auxotrophy of the S. cerevisiae BY4741 Δtkl1 mutant by plasmids harboring MmTKL1, MmTKL2, or ScTKL1 (the endogenous S. cerevisiae gene) or with no insert (pDB05). Also tested were the untransformed mutant and the wild-type parent (BY4741). Growth was tested by spotting serial dilutions at an estimated 105–100 cells/spot, left to right on parallel synthetic complete (SC) agar medium with or without added AAAs (tryptophan, tyrosine, phenylalanine) and incubating the plates at 30 °C for 3 days

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