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Table 4 Contaminations of basidiospore solutions (from Experimental set-up 1 in Table 1) with other microbes

From: Bulk isolation of basidiospores from wild mushrooms by electrostatic attraction with low risk of microbial contaminations

Fruiting body

Classic spore print (mushroom upside-top)

Basidiospores in 50 µl

Colonies on plate/50 µl spore solution plated

MEA

MEA + AB

Basidiomycete

Contaminationa

Basidiomycete

Contaminationa

Bacteria

Other fungi

Bacteria

Other fungi

Coprinellus domesticus

1.6 × 107

68

Uncountable

2 yeasts, 34 molds

51

–

122 yeasts, 14 molds

Panaeolus cinctulus

8.0 × 106

–

5.4 × 103

3 yeasts, 38 molds

–

–

8 yeasts, 14 molds

Schizophyllum commune

2.1 × 107

2563

Uncountable

34 yeasts, 4 molds

2326

–

544 yeasts, 8 molds

Tubaria hiemalis (1st mushroom)

1.9 × 104

–

1.7 × 105

14 yeasts, 6 molds

–

–

42 yeasts, 10 molds

T. hiemalis (2nd mushroom)

2.4 × 104

–

4.3 × 105

6 yeasts, 5 molds

–

–

64 yeasts, 21 molds

 

Spore print in plastic lid (mushroom upside-down)

Basidiospores in 50 µl

Colonies in plate/50 µl spore solution plated

MEA

MEA + AB

Basidiomycete

Contamination

Basidiomycete

Contamination

Bacteria

Other fungi

Bacteria

Fungi

C. domesticus

1.2 × 106

4660 (0.33% germination)

–

–

4852 (0.34% germination)

–

–

P. cinctulus

9.0 × 105

1352 (0.15% germination)

–

–

1280 (0.14% germination)

–

–

S. commune

1.8 × 106

Uncountable

–

–

Uncountable

–

–

T. hiemalis (1st mushroom)

7.8 × 103

2458 (31.5% germination)

–

–

2873 (36.8% germination)

–

–

T. hiemalis (2nd mushroom)

9.0 × 103

2213 (24.5% germination)

–

–

2437 (27.0% germination)

–

3 yeasts

  1. aNumbers of fungi in different media did not clearly correspond to each other since fast bacterial growth suppressed fungal growth and fast growing molds suppressed that of other molds and yeasts