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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Glycin-rich antimicrobial peptide YD1 from B. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid DNA of E. coli

Fig. 3

The elution profile of YD1 from a DEAE Sepharose Fast Flow ion exchange column (2.5 × 14 cm) (a) and a Sephadex G-25 permeation column (1.5 x 28 cm) (b). Tricine SDS-PAGE and in situ analysis. Lane 1, Sephadex G-25 pooled purified YD1; lane 2, protein marker (M); lane 3, 30–80% ammonium sulfate (AS) precipitation fraction; and lane 4, crude sample. Inhibition zone observed in the in situ assay is shown in the right panel (c)

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