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Table 5 Ranking of the candidate reference genes calculated using RefFinder under different conditions

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Ranking ordera All conditions Different media Different light conditions
Rice medium PD medium Czapek–Dox medium All-dark All-light 12-h light/dark
Gene Rb Gene R Gene R Gene R Gene R Gene R Gene R
1 SbLAlv9 1.68 SbLAlv9 1.32 SbJsn1 1.68 SbJsn1 1.41 SbVAC55 1.86 SbJsn1 1.68 SbLAlv9 1.57
2 SbJsn1 2.3 SbSAS1 1.41 SbtS 2.71 VAC 2.21 SbLAlv9 1.86 SbtS 2.91 SbSAS1 2.45
3 SbSAS1 2.91 SbJsn1 3.41 SbSAS1 2.83 SbSAS1 4.05 VAC 3.22 SbSAS1 2.99 SbJsn1 2.82
4 SbVAC55 3.98 SbVAC55 3.87 SbLAlv9 3.25 UBI 4.12 SbJsn1 3.94 SbLAlv9 4.16 UBI 3.44
5 SbtS 4.47 TFC 4.47 SbVAC55 5 SbLAlv9 4.53 SbSAS1 5.12 SbCHP 4.28 SbtS 5.18
6 SbCHP 5.36 SbCHP 6.34 SbTRX 5.05 SbCHP 5.76 SbCHP 5.32 SbVAC55 4.9 SbTRX 6.32
7 SbTRX 6.26 VAC 7.17 TFC 6.04 SbTRX 6.34 SbTRX 5.38 UBI 6.19 VAC 6.45
8 UBI 6.84 SbtS 7.44 UBI 6.74 TFC 7.38 SbPKI 6.84 VAC 7.5 SbVAC55 7.27
9 VAC 7.91 SbTRX 8.21 SbCHP 8.46 SbFYVE 7.44 SbtS 7.11 SbTRX 7.64 TFC 7.35
10 TFC 8.8 SbFYVE 10.47 SbFYVE 9 SbPKI 7.65 TFC 10.47 SbFYVE 8.57 SbCHP 7.61
11 SbPKI 11.17 UBI 10.74 VAC 10.47 SbVAC55 10.36 UBI 11 TFC 9.64 SbFYVE 10.74
12 SbFYVE 11.24 SbPKI 11.74 SbPKI 11.74 SbtS 10.69 SbFYVE 11.17 SbPKI 10.74 SbPKI 11.74
  1. aGenes were ranked according to their R values
  2. bThe R values were calculated by RefFinder to integrate the results from different programs, and a gene with more stable expression is expressed as a smaller number