Table 1 Primers used in this study
Primer | Sequence (5′–3′) | Purpose |
|---|---|---|
ChiF | GTTGTGGTTTGCCTGGTG | Real-time PCR |
ChiR | CCGATACTCTGCTGCTCAT | |
hphF | ATGCCTGAACTCACCGCGACGTCTG | Hygromycin amplification |
hphR | CTATTCCTTTGCCCTCGGACGAGTG | |
CF | TTCAGAGTAGGCTTTTGGATTGGT | Chi67-1 amplification |
CR | ACCCCATATTTGCTCATAATCACA | |
qdzF | ACTCGACCTGCAGGCATGCAAGCTTGAATTCCCTTGTATCTCTACACA | gpdA amplification |
qdzR | ACCAATCCAAAAGCCTACTCTGAAGGGAAAAGAAAGAGAAAAGAAAAG | |
zzzF | TGTGATTATGAGCAAATATGGGGTGGATCCACTTAACGTTACTGAAA | trpC amplification |
zzzR | GTAAACGACGGCCAGTGCCAAGCTTTCGAGTGGAGATGTGGAGTGG |