Purification and characterization of a novel glutamate dehydrogenase from Geotrichum candidum with higher alcohol and amino acid activity

AMB Express

Table 4 Effect of metal ions, chemical regents, and cofactors on the enzyme activity towards hexanol

Metal ion	Concentration (mmol/L)	Relative activity (%)^a	Metal ion	Concentration (mmol/L)	Relative activity (%)^a
K⁺	5	103.32 ± 3.24^b	Ba²⁺	5	95.35 ± 2.09
	10	121.77 ± 4.53		10	97.80 ± 0.93
	50	193.01 ± 1.45		50	97.52 ± 1.55
Zn²⁺	5	106.35 ± 1.41	Mn²⁺	5	51.85 ± 4.23
	10	105.12 ± 3.56		10	42.14 ± 2.12
	50	104.23 ± 6.46		50	12.37 ± 1.98
Mg²⁺	5	67.69 ± 5.41	Pb²⁺	5	19.84 ± 0.06
	10	69.98 ± 2.47		10	6.05 ± 2.17
	50	49.75 ± 0.58		50	4.66 ± 2.66
Fe³⁺	5	95.42 ± 0.85	Ca²⁺	5	93.47 ± 1.74
	10	89.99 ± 6.01		10	50.59 ± 1.69
	50	71.50 ± 1.19		50	44.80 ± 0.96
Fe²⁺	5	102.29 ± 3.01
	10	115.48 ± 3.40
	50	208.35 ± 0.59
NAD⁺	0.3	50.48 ± 1.05	EDTA	1.0	5.34 ± 0.03
NADH	0.3	5.29 ± 0.43	ATP	1.0	14.35 ± 0.84
NADP⁺	0.3	100	ADP	1.0	138.21 ± 3.72
NADPH	0.3	3.82 ± 0.78	DTT	1.0	30.72 ± 1.01

^aThe enzyme activity was determined by incubating the enzyme in the presence of various compounds for 60 min at 30 °C and pH 4.0 with 9.8 mmol/L hexanol as a substrate
^bValues represent the mean ± SD (n = 3) compared with the untreated control samples