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Table 4 Effect of metal ions, chemical regents, and cofactors on the enzyme activity towards hexanol

From: Purification and characterization of a novel glutamate dehydrogenase from Geotrichum candidum with higher alcohol and amino acid activity

Metal ion Concentration (mmol/L) Relative activity (%)a Metal ion Concentration (mmol/L) Relative activity (%)a
K+ 5 103.32 ± 3.24b Ba2+ 5 95.35 ± 2.09
10 121.77 ± 4.53   10 97.80 ± 0.93
50 193.01 ± 1.45   50 97.52 ± 1.55
Zn2+ 5 106.35 ± 1.41 Mn2+ 5 51.85 ± 4.23
10 105.12 ± 3.56   10 42.14 ± 2.12
50 104.23 ± 6.46   50 12.37 ± 1.98
Mg2+ 5 67.69 ± 5.41 Pb2+ 5 19.84 ± 0.06
10 69.98 ± 2.47   10 6.05 ± 2.17
50 49.75 ± 0.58   50 4.66 ± 2.66
Fe3+ 5 95.42 ± 0.85 Ca2+ 5 93.47 ± 1.74
10 89.99 ± 6.01   10 50.59 ± 1.69
50 71.50 ± 1.19   50 44.80 ± 0.96
Fe2+ 5 102.29 ± 3.01    
10 115.48 ± 3.40    
50 208.35 ± 0.59    
NAD+ 0.3 50.48 ± 1.05 EDTA 1.0 5.34 ± 0.03
NADH 0.3 5.29 ± 0.43 ATP 1.0 14.35 ± 0.84
NADP+ 0.3 100 ADP 1.0 138.21 ± 3.72
NADPH 0.3 3.82 ± 0.78 DTT 1.0 30.72 ± 1.01
  1. aThe enzyme activity was determined by incubating the enzyme in the presence of various compounds for 60 min at 30 °C and pH 4.0 with 9.8 mmol/L hexanol as a substrate
  2. bValues represent the mean ± SD (n = 3) compared with the untreated control samples