Fig. 5

Recombinant VEGF₁₆₅ induces ex vivo angiogenesis. A The CAM models were prepared using 8-day-old chick embryos treated as described in method section. Dark circles represent location of applied disks. Filter disks were soaked in (a) 0.9% NaCl; (b) 200 ng of recombinant VEGF; (c) 500 ng of recombinant VEGF; after incubation for 48 h, CAMs were photographed with a digital camera. Each group contained four CAMs and the experiment was repeated three times. B The quantitative measurement of total vessel length was performed on 50% of the total CAM surface treated in the absence or in the presence of recombinant VEGF. Significant differences: ** means p < 0.05