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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli

Fig. 1

Analysis of electrophoretic profiles. a Agarose gel profile of products resulting from PCR amplification of the VEGF transcripts present in tumor tissue: human breast cancer cell line MCF7 (lane 2), human colorectal cancer cells (lane 4) and human breast cancer cells (lane 5) and in distant-tumor tissue (lane 3) M molecular-mass marker (100 pb DNA ladder; Fermentas); lane 1 PCR negative control (sample without DNA). b Agarose gel showing PCR products from amplification of the positive clones obtained after ligation to pGEMT easy vector. Lane 1 VEGF121, lane 2/3 VEGF165, lane 4/5 VEGF189, T-: PCR negative control (sample without DNA)

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