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Table 4 Phylogenetic affiliations and number of bacterial clones obtained from retained and suspended fractions in the MFC operated for 30 days at pH 7.0

From: Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions

OTUa No. of clonesb Phylogenetic group The closest isolated strain (accession no., similarity) The closest environmental clone (accession no., similarity) Isolated source of environmental clones
Retained Suspended
1 6   Proteobacteria; Deltaproteobacteria Geobacter sulfurreducens
(NR_075009, 98.6%)
Uncultured bacterium
(GQ152932, 100%)
Mixed consortium in MFC
2 4   Proteobacteria; Gammaproteobacteria Citrobacter freundii
(AB548830, 100%)
Uncultured organism
(HQ745055, 99.9%)
Gastrointestinal specimens
3 3 1 Proteobacteria; Gammaproteobacteria Raoultella ornithinolytica
(CP004142, 100%)
Uncultured bacterium
(EF515418, 100%)
Upflow MFC anode
4 4 1 Proteobacteria; Gammaproteobacteria Pseudomonas fragi
(AB685617, 99.8%)
Uncultured bacterium
(EU469649, 99.8%)
Mammalian gut microbes
5   5 Proteobacteria; Gammaproteobacteria Aeromonas dhakensis
(KF938660, 100%)
Uncultured bacterium
(JX271966, 100%)
Activated sludge from large-scale
6   3 Proteobacteria; Gammaproteobacteria Aeromonas sharmana
(NR_043470, 96.9%)
Uncultured Aeromonas sp.
(EF679186, 98.6%)
Sewage plant
Anaerobic sludge from MFC
7 1   Proteobacteria; Betaproteobacteria Advenella kashmirensis
(KF956701, 95.1%)
Uncultured bacterium
(FN563161, 99.7%)
Mesophilic biogas digester
8 1 1 Proteobacteria; Betaproteobacteria Azospira oryzae
(DQ863512, 94.1%)
Uncultured Azospira sp.
(JF736645, 96.5%)
Biofilm on electrode in MFC
9 12 5 Bacteroidetes; Cytophagia Pontibacter korlensis
(GQ503321, 82.7%)
Bacteriodetes bacterium
(JX828412, 95.9%)
Iron-reducing enrichment culture
10 3   Bacteroidetes; Bacteroidia Bacteroides nordii
(NR_112939, 92.9%)
Uncultured bacterium
(KC179060, 99.7%)
Activated sludge
11   12 Bacteroidetes; Bacteroidia Dysgonomonas oryzarvi
(NR_113063, 99.7%)
Uncultured Dysgonomonas sp.
(JX535216, 100%)
Activated sludge from sewage
12   1 Bacteroidetes; Bacteroidia Dysgonomonas capnocytophagoides
(NR_113133, 93.2%)
Uncultured bacterium
(HQ728219, 96.1%)
Glucose-fed MFC
13 3   Firmicutes; Negativicutes Anaeroarcus burkinensis
(NR_025298, 97.7%)
Uncultured bacterium
(AB329655, 99.9%)
UASB granule
14 2   Firmicutes; Negativicutes Acidaminococcus fermentans
(NR_074928, 93.8%)
Uncultured bacterium
(JN680060, 99.5%)
Anode biofilm at psychrotolerants in MFC
15   1 Firmicutes; Clostridia Pseudoflavonifractor capillosus
(NR_025670, 89.8%)
Uncultured Clostridiales bacterium
(FJ393118, 91.0%)
Glucose-fed MFC anode
Sum 39 30     
  1. aSequences with more than 97.0% homology were considered as being the same as the operational taxonomic unit (OTU)
  2. bSuspended: the suspended fraction obtained from fermentation broth, Retained: the retained fraction obtained from anode graphite felts