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Fig. 2 | AMB Express

Fig. 2

From: Antimicrobial activity and stability of the d-amino acid substituted derivatives of antimicrobial peptide polybia-MPI

Fig. 2

The hemolytic activity of polybia-MPI and synthetic D-MPI and d-lys-MPI against human erythrocytes were determined. Fresh human blood was collected and centrifuged to remove the serum and buffy coat, and re-suspended to a final erythrocyte concentration of 8% erythrocytes suspension. Then, peptides of different concentrations from 2 to 256 μM were incubated with erythrocytes suspension for 60 min. Subsequently, the erythrocytes suspension was centrifuged and measured to monitor the release of hemoglobin at 490 nm

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