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Table 1 Identification of the isolates on the basis of conventional and molecular analysis

From: Contribution of target alteration, protection and efflux pump in achieving high ciprofloxacin resistance in Enterobacteriaceae

Isolate ID Growth characteristics on different differential and selective media Microscopic characteristics
Media Appearance Form Elevation Margin Consistency Gram-staining Size Shape Arrangement
28N, 26N, CR1, CR2, CR4, CR6, NCX9, MCX14, C6, C49, C79, C84, E8, E23, E29, E34, E36, E37, E40, E41, E42, E56, E58, G2, G3, G4 MAC DP C F E D Gram-negative S SR Single
EMB BB, GMS
XLD Y
MCX1, MCX2, MCX3, MCX4, MCX5, MCX6, NCX4 MAC P C R E G M Rd
EMB B, DC
XLD Y
NCX6, MCX10, C1, C67, E33 MAC LP C F E D M Rd
EMB B, DC C F E G
XLD Y C F E D
18, 20, 36, 44, 45, 49, 54, 58, 60,68, 74, 77, 81, 84, 94 MAC Colorless C F E D M Rd
EMB Color less C F E G
XLD Red, BC C F E D
Isolate ID Biochemical characteristics Presumptive organism ARDRA genotype
Sugar utilization tests IMViC tests Oxidase Test KIA Test Motility
Gl Su La Indole MR VP Citrate Slant Butt Gas H2S
28N, 26N, CR1, CR2, CR4, CR6, NCX9, MCX14, C6, C49, C79, C84, E8, E23, E29, E34, E36, E37, E40, E41, E42, E56, E58, G2, G3, G4 + ± + + + Y Y + + Escherichia spp. I
MCX1, MCX2, MCX3, MCX4, MCX5, MCX6, NCX4 + + + + Y Y + Enterobacter spp. II
NCX6, MCX10, C1, C67, E33 + + + + Y Y + Klebsiella spp. III
18, 20, 36, 44, 45, 49, 54, 58, 60,68, 74, 77, 81, 84, 94 + + + Red Y + Salmonella spp. IV
  1. Legends for conventional and molecular characteristics: C circular, F flat, R raised, E entire, D dry, G gummy, S short, M medium, SR short rod, Rd rod, GMS green metallic sheen, DP dark pink, BB blue black, Y yellow, P pink, B brown, R red, DC dark centered, LP light pink, Gl glucose, Su sucrose, La lactose, BC black centered
  2. Italics isolates were previously identified in our laboratory