Fig. 4

AcP degradation in crude extracts of CEN.PK113-5D and corresponding GPP1 and/or GPP2 deletions strains. The legends refer to the strains used to prepare crude cell free extracts. Protein extraction buffer (Buffer) served as a negative control. 20 mM AcP was added in the reaction mixture. Strains carrying deletions show significantly reduced AcP levels when compared to the control strain CEN.PK 113-5D. The results shown are averages from two biological replicates each performed in duplicates; error bars indicate the standard deviation