Disruption of Pseudomonas putida by high pressure homogenization: a comparison of the predictive capacity of three process models for the efficient release of arginine deiminase

AMB Express

Table 6 Validation and comparison with the unseen dataset

Statistical parameter	ADI activity (U/mL)			Total protein release (g/L)			Cell disruption (%)
Statistical parameter	RSM	ANN	SVM	RSM	ANN	SVM	RSM	ANN	SVM
Training dataset
R ²	0.970	0.869	0.975	0.963	0.854	0.960	0.984	0.945	0.991
MSE	0.255	1.175	0.208	0.029	0.151	0.031	2.725	9.298	1.586
RMSE	0.505	1.084	0.456	0.170	0.389	0.177	1.651	3.049	1.259
SEP	2.175	4.665	1.964	5.264	12.073	5.496	2.204	4.071	1.682
RPD	1.573	3.978	1.046	4.272	10.211	4.463	1.790	2.910	0.694
Unseen dataset
R ²	0.943	0.845	0.892	0.584	0.533	0.640	0.990	0.969	0.979
MSE	0.866	0.218	0.608	0.083	0.049	0.081	17.469	19.948	17.720
RMSE	0.931	0.467	0.780	0.288	0.222	0.285	4.180	4.466	4.209
SEP	3.628	1.821	3.039	8.116	6.264	8.053	6.873	7.344	6.922
RPD	3.469	1.523	2.509	5.966	5.154	5.703	6.728	6.478	5.384

R ² determination coefficient; MSE mean square error; RMSE root mean square error; SEP standard error of prediction; RPD relative percent deviation; A Cell mass concentration; B operating pressure; C number of passes