|
Run
|
A (g/L)
|
B (bar)
|
C
|
ADI activity (U/mL)
|
Total protein release (g/L)
|
Cell disruption (%)
|
|---|
|
Actual
|
RSM predicted
|
ANN predicted
|
SVM predicted
|
Actual
|
RSM predicted
|
ANN predicted
|
SVM predicted
|
Actual
|
RSM predicted
|
ANN predicted
|
SVM predicted
|
|---|
|
1
|
200
|
413.7
|
2
|
22.5
|
23.2
|
23.0
|
22.6
|
2.7
|
2.6
|
3.0
|
2.8
|
63.3
|
63.9
|
64.6
|
63.4
|
|
2
|
100
|
965.3
|
1
|
20.1
|
20.1
|
17.8
|
20.2
|
2.8
|
2.8
|
2.9
|
2.9
|
75.5
|
73.6
|
76.3
|
75.4
|
|
3
|
300
|
413.7
|
3
|
22.6
|
22.5
|
23.6
|
22.5
|
4.9
|
4.8
|
4.3
|
4.8
|
70.2
|
71.9
|
77.7
|
70.3
|
|
4
|
300
|
965.3
|
1
|
23.2
|
23.1
|
23.7
|
23.3
|
4.7
|
4.6
|
4.5
|
4.6
|
65.5
|
66.4
|
67.3
|
65.4
|
|
5
|
100
|
965.3
|
3
|
22.5
|
22.8
|
21.5
|
22.6
|
2.4
|
2.3
|
2.8
|
2.4
|
93.0
|
94.5
|
89.3
|
92.9
|
|
6
|
100
|
689.5
|
2
|
19.5
|
19.4
|
17.2
|
19.6
|
2.2
|
2.2
|
2.8
|
2.1
|
86.4
|
87.3
|
80.7
|
86.5
|
|
7
|
300
|
689.5
|
2
|
24.4
|
24.3
|
23.6
|
24.8
|
4.0
|
4.2
|
4.4
|
4.2
|
78.9
|
79.0
|
73.2
|
78.9
|
|
8
|
200
|
689.5
|
1
|
25.5
|
26.3
|
24.9
|
25.6
|
3.1
|
3.0
|
2.9
|
2.9
|
57.8
|
60.0
|
58.3
|
57.9
|
|
9
|
200
|
689.5
|
2
|
25.2
|
25.0
|
25.2
|
25.1
|
3.5
|
3.0
|
3.1
|
3.0
|
80.7
|
79.7
|
79.5
|
79.7
|
|
10
|
300
|
689.5
|
3
|
22.1
|
22.6
|
22.6
|
22.2
|
5.5
|
5.4
|
4.7
|
5.4
|
85.4
|
84.5
|
83.9
|
85.3
|
|
11
|
200
|
689.5
|
2
|
25.4
|
25.0
|
25.2
|
25.1
|
3.1
|
3.0
|
3.1
|
3.0
|
84.3
|
79.7
|
79.5
|
79.7
|
|
12
|
200
|
689.5
|
2
|
25.0
|
25.0
|
25.2
|
25.1
|
2.8
|
3.0
|
3.1
|
3.0
|
79.6
|
79.7
|
79.5
|
79.7
|
|
13
|
300
|
413.7
|
1
|
27.7
|
27.4
|
26.1
|
27.6
|
3.1
|
3.2
|
3.8
|
3.2
|
43.1
|
41.3
|
42.4
|
43.0
|
|
14
|
200
|
689.5
|
3
|
26.1
|
25.2
|
25.0
|
24.7
|
3.3
|
3.6
|
3.4
|
3.5
|
86.7
|
85.7
|
86.7
|
86.8
|
|
15
|
200
|
965.3
|
2
|
25.3
|
24.5
|
24.8
|
25.2
|
3.1
|
3.3
|
3.6
|
3.4
|
82.5
|
83.1
|
84.9
|
82.6
|
|
16
|
200
|
689.5
|
2
|
23.7
|
25.0
|
25.2
|
25.1
|
2.8
|
3.0
|
3.1
|
3.0
|
76.7
|
79.7
|
79.5
|
79.7
|
|
17
|
100
|
413.7
|
1
|
18.0
|
17.5
|
19.0
|
17.9
|
2.0
|
2.1
|
2.3
|
2.1
|
47.1
|
47.8
|
48.3
|
47.2
|
|
18
|
200
|
689.5
|
2
|
25.0
|
25.0
|
25.2
|
25.1
|
3.0
|
3.0
|
3.1
|
3.0
|
80.0
|
79.7
|
79.5
|
79.7
|
|
19
|
200
|
689.5
|
2
|
25.1
|
25.0
|
25.2
|
25.1
|
3.1
|
3.0
|
3.1
|
3.0
|
79.0
|
79.7
|
79.5
|
79.7
|
|
20
|
100
|
413.7
|
3
|
15.7
|
15.8
|
17.0
|
15.8
|
2.4
|
2.4
|
2.7
|
2.4
|
82.3
|
81.2
|
84.0
|
82.2
|
-
A, Cell mass concentration; B, Operating pressure; C, number of passes
