Table 2 PCR primers for construction of plasmids
Primers | Sequences |
|---|---|
sSN1185 | 5′-ATCCATGGTCGTCTCACTCGCGAACTGC-3′ |
sSN1186 | 5′-GTGGATCCCCAGATGCGTTTCACCCCTGC-3′ |
sSN1187 | 5′-AATCTAGAACAGGCCAAAGGTTTCAGCCTG-3′ |
sSN1188 | 5′-GATATGCATCGCCACTTTATCCAGCGGTAG-3′ |
sSN1892-accN | 5′-AAACTGCAGGGGTGAATAATCTGATTTTGTTTGACTAC-3′ |
sSN1893-accN | 5′-AAATCTAGACAGGCGTTTCACCGCCGTCTGTAAAGC-3′ |
sSN1894-tetR | 5′-TTACTAGTTTAAGACCCACTTTCACATTTAAGTTGTTTTTC-3′ |
sSN1895-tetR | 5′-AAAGGATCCTTTCTCCTCTTTAATGAATTCGGTCAGTG-3′ |
sSN1896-accORF | 5′-AAAGGATCCATGGATATTCGTAAGATTAAAAAACTG-3′ |
sSN1897-accORF | 5′-CCGACATGTTCAGATAACTTTTTACTGACGGAGC-3′ |
sSN1997aceEDox1 | 5′-TCAACATGTCTAACTACTTCAATACACTGAATCTG-3′ |
sSN1998aceEDox2 | 5′-AGACTAGTTTAACCCCCCAGTTTCGATTTATCGCG-3′ |
sSN1998aceEDox3 | 5′-AAAATGCATAGGCCTTCTCGGGCATAAGTCGGACACCATGG-3′ |
sSN1999aceEDox4 | 5′-ATTGCGAGGCTTTGTGCTTCTCTGG-3′ |
sSN2000aceEDox5 | 5′-CCCATGGGGATCCAGGCCTTCTAGAACT-3′ |
sSN2001aceEDox6 | 5′-GGGGTACCTGAGCAAACTGGCCTCA-3′ |