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Table 2 PCR primers for construction of plasmids

From: Pyruvate production using engineered Escherichia coli

Primers Sequences
sSN1185 5′-ATCCATGGTCGTCTCACTCGCGAACTGC-3′
sSN1186 5′-GTGGATCCCCAGATGCGTTTCACCCCTGC-3′
sSN1187 5′-AATCTAGAACAGGCCAAAGGTTTCAGCCTG-3′
sSN1188 5′-GATATGCATCGCCACTTTATCCAGCGGTAG-3′
sSN1892-accN 5′-AAACTGCAGGGGTGAATAATCTGATTTTGTTTGACTAC-3′
sSN1893-accN 5′-AAATCTAGACAGGCGTTTCACCGCCGTCTGTAAAGC-3′
sSN1894-tetR 5′-TTACTAGTTTAAGACCCACTTTCACATTTAAGTTGTTTTTC-3′
sSN1895-tetR 5′-AAAGGATCCTTTCTCCTCTTTAATGAATTCGGTCAGTG-3′
sSN1896-accORF 5′-AAAGGATCCATGGATATTCGTAAGATTAAAAAACTG-3′
sSN1897-accORF 5′-CCGACATGTTCAGATAACTTTTTACTGACGGAGC-3′
sSN1997aceEDox1 5′-TCAACATGTCTAACTACTTCAATACACTGAATCTG-3′
sSN1998aceEDox2 5′-AGACTAGTTTAACCCCCCAGTTTCGATTTATCGCG-3′
sSN1998aceEDox3 5′-AAAATGCATAGGCCTTCTCGGGCATAAGTCGGACACCATGG-3′
sSN1999aceEDox4 5′-ATTGCGAGGCTTTGTGCTTCTCTGG-3′
sSN2000aceEDox5 5′-CCCATGGGGATCCAGGCCTTCTAGAACT-3′
sSN2001aceEDox6 5′-GGGGTACCTGAGCAAACTGGCCTCA-3′
  1. The restriction enzyme site is underlined