Fig. 5From: Bioscreening and expression of a camel anti-CTGF VHH nanobody and its renaturation by a novel dialysis–dilution methodThe expression and purification of the VHH nanobody from E. coli. Lane M marker; lane 1 precipitates after cell lysis; lane 2 supernatant after cell lysis; lane 3 elution of Ni–NTA chromatography; lane 4 the VHH antibody renatured by the dialysis–dilution method; lane 5 the VHH antibody desalted by ultra-filtrationBack to article page