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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Expression, purification and characterization of an endoglucanase from Serratia proteamaculans CDBB-1961, isolated from the gut of Dendroctonus adjunctus (Coleoptera: Scolytinae)

Fig. 2

Protein and zymogram analysis of Spr Cel8A in 10 % SDS-PAGE. a Analysis of total protein extracts of transformed E. coli M15 [pREP4] /pQE30/spr cel8A, M: MW protein standards; line 1 and 2 non-induced soluble fraction medium and bacterial cell harvested respectively; line 3 induced soluble fraction medium; line 4 and 5 induced lysed bacterial cells soluble fraction. b Purification of recombinant Spr Cel8A; line 1 total protein extracts of transformed E. coli M15 [pREP4] /pQE30/Spr Cel8A and line 2 Spr Cel8A purified. c Zimogram analysis of Spr Cel8A in 10 % SDS-PAGE with 2 % CMC

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