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Table 1 Yeast strains and plasmids used in this study

From: Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells

Name description Description Reference source
Yeast strains
BY4742 MATα his3∆1 ura3∆0 leu2∆0 lys2∆0 Brachmann et al. (1998)
MCF4741 MAT a his3∆1 ura3∆0 leu2∆0 met15∆0 Fig. 1::Fig. 1-EGFP-loxP-kanMX4-loxP Fukuda et al. (2013b)
HR42-11T MATα his3∆1 ura3∆0 leu2∆0 lys2∆0 hmr a inc ::HMR a cut -loxP-kanMX4-loxP Fukuda et al. (2013b)
K6 Sake yeast; Kyokai No. 6; MAT a/ α NBRC2346a
K7 Sake yeast; Kyokai No. 7; MAT a/ α NBRC2347a
K9 Sake yeast; Kyokai No. 9; MAT a/ α NBRC2377a
K6A a-type of strain derived from Kyokai No. 6 Present study
K6AL α-type of strain derived from Kyokai No. 6 Present study
K7A a-type of strain derived from Kyokai No. 7 Present study
K7AL α-type of strain derived from Kyokai No. 7 Present study
K9A a-type of strain derived from Kyokai No. 9 Present study
K9AL α-type of strain derived from Kyokai No. 9 Present study
K67 Hybrid strain generated by zygosis of K6A and K7AL Present study
K69 Hybrid strain generated by zygosis of K6A and K9AL Present study
K76 Hybrid strain generated by zygosis of K7A and K6AL Present study
K79 Hybrid strain generated by zygosis of K7A and K9AL Present study
K96 Hybrid strain generated by zygosis of K9A and K6AL Present study
K97 Hybrid strain generated by zygosis of K9A and K7AL Present study
K76A a-type of strain derived from K76 Present study
K76AL α-type of strain derived from K76 Present study
K76x9 Hybrid strain generated by zygosis of K76A and K9AL Present study
Plasmids
pLY-hygro 2μ ori, LEU2 marker and P TDH3 -hygro Present study
pHY-kan 2μ ori, HIS3 marker and P TEF1 -kanMX4 Present study
pAUR112 CEN4/ARS1 ori, URA3, AUR1-C Takara Bio, Inc., Shiga, Japan
pLhyS-2 K-Pa1 CEN6/ARSH4 ori, LEU2, P TDH3 -hygro, P STE2 -kanMX4 and P PGK1 -a1 Present study
pHhyS-3 K-2α CEN6/ARSH4 ori, HIS3, P TDH3 -hygro, P STE3 -kanMX4 and P STE2 -α2 Present study
  1. aResources were provided by Biological Resource Center (NBRC), NITE, Japan