Table 1 Yeast strains and plasmids used in this study
From: Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells
Name description | Description | Reference source |
|---|---|---|
Yeast strains | ||
BY4742 | MATα his3∆1 ura3∆0 leu2∆0 lys2∆0 | Brachmann et al. (1998) |
MCF4741 | MAT a his3∆1 ura3∆0 leu2∆0 met15∆0 Fig. 1::Fig. 1-EGFP-loxP-kanMX4-loxP | Fukuda et al. (2013b) |
HR42-11T | MATα his3∆1 ura3∆0 leu2∆0 lys2∆0 hmr a inc ::HMR a cut -loxP-kanMX4-loxP | Fukuda et al. (2013b) |
K6 | Sake yeast; Kyokai No. 6; MAT a/ α | NBRC2346a |
K7 | Sake yeast; Kyokai No. 7; MAT a/ α | NBRC2347a |
K9 | Sake yeast; Kyokai No. 9; MAT a/ α | NBRC2377a |
K6A | a-type of strain derived from Kyokai No. 6 | Present study |
K6AL | α-type of strain derived from Kyokai No. 6 | Present study |
K7A | a-type of strain derived from Kyokai No. 7 | Present study |
K7AL | α-type of strain derived from Kyokai No. 7 | Present study |
K9A | a-type of strain derived from Kyokai No. 9 | Present study |
K9AL | α-type of strain derived from Kyokai No. 9 | Present study |
K67 | Hybrid strain generated by zygosis of K6A and K7AL | Present study |
K69 | Hybrid strain generated by zygosis of K6A and K9AL | Present study |
K76 | Hybrid strain generated by zygosis of K7A and K6AL | Present study |
K79 | Hybrid strain generated by zygosis of K7A and K9AL | Present study |
K96 | Hybrid strain generated by zygosis of K9A and K6AL | Present study |
K97 | Hybrid strain generated by zygosis of K9A and K7AL | Present study |
K76A | a-type of strain derived from K76 | Present study |
K76AL | α-type of strain derived from K76 | Present study |
K76x9 | Hybrid strain generated by zygosis of K76A and K9AL | Present study |
Plasmids | ||
pLY-hygro | 2μ ori, LEU2 marker and P TDH3 -hygro | Present study |
pHY-kan | 2μ ori, HIS3 marker and P TEF1 -kanMX4 | Present study |
pAUR112 | CEN4/ARS1 ori, URA3, AUR1-C | Takara Bio, Inc., Shiga, Japan |
pLhyS-2 K-Pa1 | CEN6/ARSH4 ori, LEU2, P TDH3 -hygro, P STE2 -kanMX4 and P PGK1 -a1 | Present study |
pHhyS-3 K-2α | CEN6/ARSH4 ori, HIS3, P TDH3 -hygro, P STE3 -kanMX4 and P STE2 -α2 | Present study |