Fig. 2
Detection of PP-V in extracts of P. purpurogenum. a TLC of the extracellular and intracellular pigment. b HPLC chromatogram in extracellular pigment from basal and 10 mM glutamine medium. Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL of basal medium or basal medium with addition of glutamine (f.c. 1 or 10 mM l-glutamine) for 72–96 h at 30 °C with shaking at 200 rpm. The mycelia were harvested and immersed in methanol for 24 h. This methanol extract was filtered through filter paper and evaporated to dryness in vacuo. Pigments were detected by TLC using a silica gel 60 plate with the developing solvent mixture n-BuOH: AcOH: H2O (12:3:5). The extracellular PP-V was detected by HPLC (570 nm). The filtrates derived from basal and 10 mM glutamine medium for 96 h was used as samples. PP-V that was purified was used as the internal control