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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients

Fig. 4

The OmpA-like BCAL2958 protein is immunoreactive with sera from CF patients infected with Bcc. a Western blotting of the purified recombinant protein BCAL2958 from B. cenocepacia J2315 probed with the Human serum samples SCF1, SCF2, SCF3 and SCF4 from CF patients infected with Bcc bacteria, or a pool of human serum sample from healthy donors SH. The BSA protein was used in the assay as a negative control. Lanes M—Precision Plus Protein™ Dual Xtra Standard (BIO-RAD); 1—purified recombinant BCAL2958 protein; 2—albumin bovine fraction V (BSA, Nzytech). b IgG antibody levels present on sera from CF patients infected with Bcc (SCF1, 2, 3 and 4) and in healthy individuals (SH) against BCAL2958 protein. Serum antibody concentrations were defined as endpoint titers and were calculated as the reciprocal of the highest serum dilution producing an OD450 nm above cutoff value. The cutoff value was determined as the mean OD450 nm of the corresponding dilution of control sera plus 3 standard deviations (dashed line). A titer of ≥1000 was considered as positive

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