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Table 2 Total fatty acid (FA) content and composition as % of total fatty acids (g g−1) in cells of R. opacus B4 and R. opacus PD630

From: Rhodococcus opacus B4: a promising bacterium for production of biofuels and biobased chemicals

Strain

Substrate

Time (h)

FA (g g-1 CDW)

Relative proportion of fatty acids [%, w/w]

C10:0

C12:0

C14:0

C16:0

C16:1

C17:0

C17:1

C18:0

C18:1

R. opacus B4

Glucose

0

0.364a

–

–

1.7

33.6

5.7

36.6

8.2

4.4

9.9

24

0.528 ± 0.049

–

–

2.0 ± 0.16

38.5 ± 0.74

5.0 ± 0.44

22.9 ± 0.58

15.0 ± 0.09

2.8 ± 0.34

13.7 ± 0.34

72

0.613 ± 0.051

–

–

1.6 ± 0.04

35.6 ± 0.19

4.5 ± 0.01

22.4 ± 1.21

16.5 ± 0.47

3.8 ± 0.27

15.6 ± 0.27

Acetate

0

0.235a

–

–

2.2

44.5

3.8

14.8

7.6

14.2

12.2

24

0.664 ± 0.013

–

–

1.4. ± 0.04

33.4 ± 0.43

4.1 ± 0.08

26.5 ± 1.00

20.3 ± 0.94

0.91 ± 0.15

13.4 ± 1.06

72

0.517 ± 0.014

–

–

1.3 ± 0.03

29.9 ± 0.79

4.4 ± 0.05

25.5 ± 0.63

22.8 ± 0.56

–

16.1 ± 0.84

Hexadecane

0

0.087a

–

–

1.1

54.0

37.2

–

–

–

4.2

24

0.273 ± 0.088

–

–

4.3 ± 0.55

48.3 ± 8.9

45.8 ± 11.71

–

–

–

3.2 ± 0.72

72

0.285 ± 0.037

4.8 ± 0.497

0.6 ± 0.009

4.2 ± 0.15

54.4 ± 0.18

31.5 ± 3.41

–

–

2.6 ± 0.49

3.1 ± 1.05

R. opacus PD630

Glucose

0

0.525a

–

–

1.6

38.1

9.6

22.4

16.2

3.8

8.3

24

0.507 ± 0.013

–

–

1.4 ± 0.04

37.6 ± 0.67

11.6 ± 0.45

12.8 ± 0.31

19.5 ± 0.39

2.3 ± 0.43

14.8 ± 0.41

72

0.580 ± 0.014

–

–

1.3 ± 0.08

33.3 ± 0.11

11.8 ± 0.31

15.9 ± 0.04

21.4 ± 0.20

2.4 ± 0.12

14.8 ± 0.17

Acetate

0

0.238a

–

–

1.9

39.5

17.6

9.6

19.3

–

12.1

24

0.505 ± 0.006

–

–

1.6 ± 0.41

36.8 ± 3.45

10.7 ± 1.64

15.4 ± 1.19

22.1 ± 3.52

6.8 ± 0.97

10.0 ± 1.46

72

0.449 ± 0.001

–

–

1.3 ± 0.12

30.5 ± 0.87

12.3 ± 0.12

15.9 ± 0.04

28.1 ± 0.78

–

11.8 ± 0.02

Hexadecane

0

0.116a

–

–

3.2

35.3

59.1

–

–

–

2.4

24

0.218 ± 0.003

–

–

4.1 ± 0.38

53.6 ± 4.21

42.4 ± 038

–

–

–

–

72

0.254 ± 0.041

–

0.5 ± 0.009

3.7 ± 0.06

51.7 ± 1.27

48.9 ± 1.47

–

–

–

2.0 ± 0.37

  1. Cells were cultivated in different carbon sources under nitrogen limiting conditions (C/N = 300) during 24 and 72 h
  2. The means are the result of at least two independent experiments, ± s.d
  3. aCorresponds to one replicate
  4. – Not detected or <0.5 %