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Table 1 Cloning of different bacterial β-Xylosidase genes in E. coli

From: Molecular cloning and comparative sequence analysis of fungal β-Xylosidases

Source organism Gene Vector Host Molecular mass of the recombinant enzyme (kDa) Characteristics of the recombinant enzyme Reference
C. stercorarium F-9 Xyl43B pYK306, pT7Blue T, pET-28a E. coli JM109, E. coli DH5α 56.3 Temperature = 80 °C pH 3.5 enzyme activity = 10 U mg − 1stability 15 min at 50–70 °C Km = 6.2 mm Vmax = 15 μmol min−1 mg−1 Suryani et al. (2004)
C. stercorarium F-9   pBR322 E. coli JM109 Temperature = 80 °C enzyme activity = 8.16 U mg −1 Sakka et al. (1990)
Butyrivibrio fibrisolvens GS113 xylB pUC18 E. coli DH5α 60 Specific activity 0.2 nmol min−1 mg−1 Utt et al. (1991)
B. stearothermophilus 21 xylA pUC19 E. coli JM109 75 Enzyme activity 1.56 U 10 mL−1 Baba et al. (1994)
Thermoanaerobacterium sp. JW/SL YS485 xylB pUC18 E. coli TG-1 58.5 Temperature = 65 °C pH = 6.0 specific activity = 0.53 U mg −1 Lorenz and Wiegel (1997)
Thermotoga thermarum DSM 5069 XynB3 pET-20b E. coli Top10 and BL21 (DE3) 85 kD Temperature = 95 °C pH 6.0 specific activity = 116 U mg −1Km = 0.27 mM Vmax = 223.2 U mg 1 Kcat/Km 1173.4 mM−1 s−1 Shi et al. (2013)
P. ruminicola B14 xynB pUC19/L3 E. coli DH 5α pH = 6.5 enzyme activity = 540 nmol min−1 mg−1 Gasparic et al. (1995)
C. saccharolyticum Tp8T6.3.3.1 aryl pBR322 E.coli 53 Temperature = 70 °C pH = 5.7 specific activity = 49.2 µmol min−1 mg−1 km = 10 mM Vmax = 64 U mg−1 Hudson et al. (1991)
Bifidobacterium breve K-110 XylBK pGEM-T E. coli BL21(DE3) JM109(DE3) 55.7 Temperature = 45 °C pH = 6.0 specific activity = 3.32 Umg−1 km = 1.45 mM Vmax = 10.75 μmol min−1 mg−1 Hyun et al. (2012)
Thermobifida fusca Xyl43A pIJ702 E. coli BL21(DE3)/pLysS Temperature = 55–60 °C pH = 5.5–6.0 km = 0.23 mM Kcat = 4.83 s−1 Morais et al. (2011)
Ruminococcus albus 8 Xyl3A pET-46b E. coli JM109 77.3   Moon et al. (2011)
S. thermoviolaceus OPC-520 bxlA pUC18 pUC19 E. coli JM109 82 Optimum temperature = 50 °C optimum pH = 6.5 stable at 50 °C activity lost at 60 °C Tsujibo et al. (2001)
Thermoanaerobacterium saccharolyticum B6A-RI xynB pHC79 (BRL) E. coli DH5a 60 Optimum temperature = 65 °C loss of activity at 85 °C half-life = 55 min at 75 °C Lee and Zeikus (1993)
Thermoanaerobacterium saccharolyticum JW/SL-YS485 XylC pMD19-T E. coli JM109 78 Temperature = 65 °C pH = 6.0 specific activity = 45.8 U mg−1 half-life = 1 h at 67 °C Km = 28 mM Vmax = 276 U mg−1 Shao et al. ( 2011)
P. woosongensis KCTC 3953 (DSM 16971) XylC pUC19 pET23a(+) E. coli DH5α E. coli BL21(DE3) 55 Temperature = 95 °C pH = 6.5 specific activity = 659.9 mU mg−1 protein Km = 8.5 mM Vmax = 3.1 µmol min−1mg−1 half-life = 22 min at 95 °C Kim and Yoon (2010)
Butyrivibrio fibrisolvens GS113 xylB pUC18 E. coli DHS5α 60 Temperature = 60 °C pH = 5.5 specific activity = 0.13 µmol min−1 mg−1 Sewell et al. (1989)
B. halodurans C-125 Bxyl pQEBxyl E coli JM109 61 Temperature = 45 °C pH = 7.0 enzyme activity = 7811.1 mU Km = 1.9 mmol L−1 Vmax = 0.65 µmol min−1 mg−1 Liang et al. (2009)
Vibrio sp. XY-214 xloA pBluescriptII KS (−) E. coli DH5α 60 Temperautre = 35 °C pH = 7.0 km = 0.244 mM Vmax = 1.82 µmol min-1 mg−1 Umemoto et al. (2008)