Table 2 Sequences of the primers used in this study
From: Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli
Primer | Sequence (5′–3′) | Restriction sitea |
|---|---|---|
bktB-FwEcoRI | CCGGAATTCAAGGAGGAATAAATGACGCGTGAAGTGGTAGTGG | EcoRI |
bktB-RvXbaI | TGCTCTAGAGCTCAGATACGCTCGAAGATGGCG | XbaI |
pct-FwBamHI | CGCGGATCCAATGCGCAAGGTGGAGATTATC | BamHI |
pct-RvEcoRI | CCGGAATTCTCACTTCTTCAGGCCCATCGG | EcoRI |