Fig. 3
Growth kinetics of Brc in the presence or absence of the originating biofilms, under agitation or static conditions. Twenty-four hours-old biofilms were incubated with TSB0.65 %G for 180 min under agitation (a) or static conditions (b). Every 60 min an aliquot was collected and the number of total or cultivable cells was determined, respectively, by flow cytometry and by CFU counting. The dots represent the average and error bars the standard deviation of 2–3 independent experiments. Statistical significance was determined using Unpaired T test (Two-tailed) with Welch’s correction. *P < 0.05, **P < 0.01, ***P < 0.001. The section c illustrates the experimental set-up of the assay performed to obtain the results depicted in panels a and b. BF biofilms