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AMB Express

Table 2 Effect of additives on NecC activity

From: Characterization of a novel oxidase from Thelonectria discophora SANK 18292 involved in nectrisine biosynthesis

Additives

Relative activity (%)

None

100

D-sorbitol

98

D-arabinitol

97

Xylitol

101

NaCl

97

MgCl2

102

KCl

101

CaCl2

100

MnCl2 (0.2 mM)

120

MnCl2 (1 mM)

127

MnCl2 (5 mM)

130

CoCl2

92

NiCl2

89

CuCl2

73

ZnCl2

99

EDTA

40

  1. The enzyme activities were measured under standard assay conditions containing 1 mM 4-amino-4-deoxyarabinitol with 1 mM per additives, unless otherwise specifically noted. Values are an average of three independent experiments
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