From: Characterization of a novel oxidase from Thelonectria discophora SANK 18292 involved in nectrisine biosynthesis
Purification step
Total protein
(mg)
Total activity
(U)
Specific activity
(U/mg)
Yield
(%)
Purification
(fold)
Mycelium extract
363.9
40.7
0.11
100
1.0
Ammonium sulfate precipitation
49.4
43.1
0.87
106
7.8
DEAE chromatography
14.9
10.0
0.67
25
6.0