Fig. 6

Effect of pH, temperature and 4-amino-4-deoxyarabinitol concentration on NecC activity. a The enzyme activity was measured under standard assay conditions except for pH. closed square, citrate/phosphate buffer; closed triangle, phosphate buffer; closed circle, glycine/NaOH buffer. b The enzyme activity was measured under standard assay conditions at various temperatures (closed circle). For the stability study, the enzyme in 50 mM potassium phosphate buffer pH 7.0 was preincubated for an hour at the indicated temperature, then the remaining activity was measured under the standard condition (closed triangle). c DSC thermogram of NecC in PBS, pH 7.4. d The enzyme activity was measured under standard assay conditions except for 4-amino-4-deoxyarabinitol concentration