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Table 1 Conversion of pNO2PheDU ((R/S)-4a) during 24 h whole cell biocatalysis with recombinant E. coli JM109 expressing the hydantoinase of A. crystallopoietes

From: Chemical synthesis and enzymatic, stereoselective hydrolysis of a functionalized dihydropyrimidine for the synthesis of β-amino acids

Time (h)

pNO2PheDUa (mM)

Conversion (%)

0

1.94 ± 0.03

–

1

1.63 ± 0.05

16

2

1.29 ± 0.04

34

3

1.24 ± 0.02

36

4

1.18 ± 0.04

39

5

1.16 ± 0.11

40

24

0.54 ± 0.05

72

  1. aInitial substrate concentration: 2 mM. Reactions and measurements were carried out in triplicates. Detection by HPLC Agilent 1200 system (HyperClone ODS-C18 column; 257 nm, 22 °C, 0.8 mL/min; initial mobile phase: 5 % (v/v) acetonitrile acidified with 0.5 % (v/v) trifluoroacetic acid and 95 % (v/v) bidest. water, 0-25 min: acetonitrile ratio increased to 10 % (v/v), 25–26 min acetonitrile ratio lowered to 5 % (v/v)
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