Fig. 3
From: Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Plate assay for detection of cellulase activity in transformed P. pastoris cells. CMC agar plates were stained with Congo Red and enzyme activity is detected by the presence of a halo around the colony. a P. pastoris colonies grown for 72 h under methanol induction. b Supernatants (2 μL) from induced cultures collected at different times spotted directly onto CMC plate. (1) Recombinant yeast transformed with pPIC9 (negative control); (2) clone FUSAOX