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Table 2 Experimental variables at different levels used for production of Melanin

From: Improved production of melanin from Aspergillus fumigatus AFGRD105 by optimization of media factors

Factors Variable Units Experimental values
Low (−1) High (+1)
1 pH   5 9
2 Temperature °C 40 50
3 Inoculum volume CFU/Ml 0.5 1.5
4 Incubation time Days 3 7
5 Substrate g 3 7
6 Moisture content % 10 25
7 Sodium dihydrogen phosphate g 10 14
8 Potassium dihydrogen phosphate g 1 3
9 Magnesium sulphate g 0.1 0.5
10 Calcium chloride g 0.5 1.0
11 Ferrous sulphate g 0.1 1