Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes

AMB Express

Table 1 List of primers

Primer	Sequence (5′→3′)	Restriction site
SP1	AGAGCATGCGTAGGAGAAAATAAAATGAAAAAGATTTGGCTGGCGCTGGCTGG	–
SP2	TCTTTGTAGTCCGCCGATGCGCTAAACGCTAAAACTAAACCAGCCAGCGCCAGCC	–
SP3	CCCCAAGCTTGGGAGAGCATGCGTAGGAG	Hind III
SP4	(GA)₁₀ GGCCGGCTGGGCCCCTTTGTAGTCCGCCG	Sfi I
SupASC	GGAACCGCGTGCCGCACAGACTGTTGAAAGTTG	–
FFTcM13	(GA)₁₀ GGCCCAGCCGGCCGGAGAAAGAATAGAAACTGG	Sfi I
RFTcM13	ATAGTTTAGCGGCCGCTGGAATAAATTCAAATCTGTC	Not I
FDIIc	TCACGGATCCAGAGAAATTTATACAAACCCAGTATTAGAAAAT	Bam HI
RDIIc	TATAAAGCTTACTACGATGTATCCAAGAGAACATAGGAGCTCTTATTATACT	Hind III
3Aup	(GA)₁₀ GGCCCAGCCGGCCAAAGATGTCATTCAAAAAGGC	Sfi I
3Alow	(GA)₁₀ GCGGCCGCATTCACTGGAATAAATTCAATTTTGTC	Not I
Pro-Sf1	(GA)₁₀ GGCCCAGCCGGCCATGGAAAATTTAAATCATTGTCC	Sfi I
Pro-Not	ATAGTTTAGCGGCCGCGAGGGTTCCATTAATAGCGC	Not I
T7B	ATACGAAATTAATACGACTCACTATAGGGAGACCACAACGG	–
SDMRGS	AGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATATCCATGAGAGGATCG	–
TolAkurtz	CCGCACACCAGTAAGGTGTGCGGTTTCAGTTGCCGCTTTCTTTCT	–
FFTc	TCACGGATCCGGAGAAAGAATAGAAACTGG	Bam HI
RFTc	TATAAAGCTTTGGAATAAATTCAAATCTGTC	Hind III
CytFRD	CACGGATCCCCAAATGAAATCAATAATCTTC	Bam HI
CytRRD	ATAAAGCTTTGGTTGTGCAAATTTCAAAGATTG	Hind III