Fig. 2
From: Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes
a Western blot analysis of 1011 M13 phages displaying Cry1Ac toxin prepared from E. coli cells harbouring the phagemids pCANTAB 5E-Cry1Ac (lane 2), pCAD-Cry1Ac (lane 3) or pCADS-Cry1Ac (lane 4). Lane 1 is trypsin-activated Cry1Ac toxin and lane 5 is the M13 helper phage. Values above the bands indicate the number fold of Cry1Ac displaying level as judged by the optical density of the 100 kDa band. b ELISA binding assays of M13-Cry1Ac phage particles, prepared from the phagemid pCAD-Cry1Ac, to cadherin fragment CR7-12. c Analysis of binding competition by ELISA of M13-Cry1Ac in presence of increasing concentrations of Cry1Ac toxin. d Toxicity of Cry1Ac-M13 (1011) to neonates M. sexta larvae after 7 days. Error bars at each plot represent the standard deviation