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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Fig. 4

SDS-PAGE showing an expression of GluDH-BA in E. coli BL21(DE3). Lane; M, protein marker (PageRuler™ Prestained Protein Ladder, Thermo Scientific); 1 whole-cell without an induction; 2 whole-cell grown in an autoinduction medium at 37 °C for 6 h and then at 20 °C for 14 h; 3 soluble fraction prepared from 2; and 4 purified GluDH-BA eluted from Ni-column. Lane 3 and 4 contained 10 µg of protein per lane

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