Antibacterial efficacy of ultrahigh molecular weight polyethylene with silver containing diamond-like surface layers

Harrasser, Norbert; Jüssen, Sebastian; Banke, Ingo J.; Kmeth, Ralf; von Eisenhart-Rothe, Ruediger; Stritzker, Bernd; Gollwitzer, Hans; Burgkart, Rainer

doi:10.1186/s13568-015-0148-x

AMB Express

Table 1 Physical parameters of DLC conversion and antibacterial effect of different Ag-DLC-PE surfaces compared to untreated PE

From: Antibacterial efficacy of ultrahigh molecular weight polyethylene with silver containing diamond-like surface layers

Implantation energy, fluence	Surface adhesion (CFU; mean ± SD)	Bacterial growth of Ag-DLC-PE (log-levels^a/%)^b	p values	Planktonic growth (CFU/ml; mean ± SD)	Bacterial growth of Ag-DLC-PE (log-levels^a/%)^b	p values
Constant fluences (1st testing group)
100 keV. 1 × 10¹⁷ cm⁻²	3.2 × 10⁴ ± 3.3 × 10⁴	+0.25/+77.8 %	0.901	4.5 × 10⁵ ± 3.0 × 10⁵	+0.5/+200 %	<0.05
80 keV. 1 × 10¹⁷ cm⁻²	1.7 × 10⁴ ± 1.4 × 10⁴	−0.02/−5.6 %	0.252	2.8 × 10⁵ ± 1.3 × 10⁵	+0.3/+86.7 %	<0.05
60 keV. 1 × 10¹⁷ cm⁻²	2.6 × 10³ ± 2.5 × 10³	−0.8/−85.6 %	<0.05	1.7 × 10⁵ ± 8.5 × 10⁴	+0.05/+13.3 %	0.884
Untreated PE	1.8 × 10⁴ ± 9.4 × 10³			1.5 × 10⁵ ± 2.8 × 10⁴
Constant implantation energies (2nd testing group)
10 keV. 1 × 10¹⁶ cm⁻²	2.7 × 10⁴ ± 9.8 × 10³	−0.03/−6.9 %	0.821	6.1 × 10⁵ ± 2.0 × 10⁵	+0.3/+103.3 %	<0.05
10 keV. 5 × 10¹⁶ cm⁻²	6.8 × 10³ ± 6.2 × 10³	−0.6/−76.6 %	<0.05	1.4 × 10⁵ ± 1.1 × 10⁵	−0.3/−53.3 %	<0.05
10 keV. 1 × 10¹⁷ cm⁻²	9.6 × 10² ± 8.5 × 10²	−1.5/−96.7 %	<0.05	1.5 × 10⁴ ± 1.2 × 10⁴	−1.3/−95 %	<0.05
Untreated PE	2.9 × 10⁴ ± 2.0 × 10⁴			3.0 × 10⁵ ± 6.5 × 10⁴
Fluence vs. implantation energies (3rd testing group)
20 keV. 5 × 10¹⁶ cm⁻²	4.0 × 10³ ± 3.5 × 10³	−1.0/−89.2 %	<0.05	5.8 × 10⁴ ± 1.9 × 10⁴	−0.4/−55.4 %	<0.05
20 keV. 1 × 10¹⁷ cm⁻²	2.0 × 10³ ± 1.6 × 10³	−1.3/−94.6 %	<0.05	3.0 × 10⁴ ± 1.3 × 10⁴	−0.6/−76.9 %	<0.05
10 keV. 5 × 10¹⁶ cm⁻²	3.9 × 10³ ± 2.8 × 10³	−1.0/−89.6 %	<0.05	6.4 × 10⁴ ± 4.8 × 10⁴	−0.3/−50.8 %	<0.05
10 keV. 1 × 10¹⁷ cm⁻²	2.3 × 10³ ± 1.9 × 10³	−1.2/−93.8 %	<0.05	3.6 × 10⁴ ± 2.3 × 10⁴	−0.6/−72.3 %	<0.05
Untreated PE	3.7 × 10⁴ ± 2.8 × 10⁴			1.3 × 10⁵ ± 3.4 × 10⁴

fluence amount of ions received by a surface per unit area (ions/cm²), CFU colony forming units, SD standard deviation
^alog-levels = bacterial counts calculated as shown in following equation: log-levels = log₁₀(CFU of Ag-DLC-PE) − log₁₀(CFU of untreated PE)
^bPositive values (log-levels/%) express increased bacterial growth on Ag-DLC-PE compared to PE, negative values express reduced bacterial growth on Ag-DLC-PE compared to PE

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