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Table 2 Comparison of process parameters during fermentation of Bacillus subtilis DSM 10T employing medium B and C

From: Enhancement of Surfactin yield by improving the medium composition and fermentation process

  Fed-batch Batch
The current study Willenbacher et al. (2014)
Applied medium C B
Fermentation approach Foam fractionation Foam fractionation
Initial glucose conc. 8 g/L 40 g/L
Addition of glucose 23 mL of 450 g/L
Final glucose conc. 0 g/L 29.19 g/L
Cultivation time (h) 34 30
Max. CDW (g/L) 3.80 2.97
µmax (h−1) 0.31 0.34
Max. cSurfactin foam (g/L) 3.67 3.99
Foam volume (mL) 435 334
Surfactin in foam (g) 1.02 0.74
Overall Surfactin (g) 1.22 0.81
YP/X (g/g) 0.26 0.19
YX/S (g/g) 0.20 0.27
YP/S (g/g) 0.05 0.03
Int. qSurfactin [g/(g h)] 0.009 0.006
Int. vol. qSurfactin [g/(L h)] 0.022 0.017
Overall Surfactin recovery (%) 83.81 91.96
Max. Surfactin enrichment 27.10 101.92
Mean bacterial enrichment 0.41 1.60
  1. The approach and results of B. subtilis DSM 10T batch fermentation (Willenbacher et al. 2014) is compared to data collected during fed-batch fermentation of B. subtilis DSM 10T employing the further optimized medium C