Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its’ pharmacological potential

AMB Express

Table 2 Biochemical and physiological characteristics of Streptomyces spp. AJ8 isolated from Kovalam solar salt works in comparison with reference Streptomyces strains

Sl. no	Confirmative tests	AJ8^a	Reference strains
Sl. no	Confirmative tests	AJ8^a	AJ9^a	RJ1^b	RJ2^c
1	Grams staining	+	+	+	+
2	Motility	Non-motile	Non-motile	Motile	Motile
	Biochemical tests
3	Methyl Red (MR)	+	+	+	–
4	Voges Proskauer (VP)	–	–	–	–
5	Indole production	–	–	–	–
6	Nitrate reduction	+	+	+	+
7	TSI test	+	+	+	+
8	H₂S production	–	–	–	–
9	Urease activity	–	–	–	–
10	Starch hydrolysis	+	–	+	–
Carbon source (1 % w/v)
11	Glucose	+	+	+	+
12	Sucrose	+	+	+	+
13	Maltose	+	+	+	+
14	Mannitol	+	+	+	+
15	Starch	+	+	+	–
16	Sorbitol	+	+	+	–
Nitrogen sources (1 % w/v)
17	Histidine	++	+	+	+
18	Valine	++	+	++	+
19	Alanine	++	+	+	+
20	Methionine	+	+	++	+
21	Tryptophan	+	+	+	+
Effect of pH
22	5.5	+	+	+	+
23	6.5	+	+	+	+
24	7.5	+	+	++	++
25	8.5	++	++	+	+
26	9.5	+++	+	–	–
27	10.5	+	–	–	–
Effect of NaCl concentration (w/v)
28	2	++	+	++	++
29	4	++	+	++	++
30	6	+++	++	+++	+++
31	8	++++	+++	++	++
32	10	++	++	+	+

^a Streptomyces spp. AJ8
^b Streptomyces spp. AJ9: Jenifer et al. (2013); Streptomyces spp. RJ1
^c Streptomyces spp. RJ2: Remya (2013). All reference strains were isolated from solar salt works