AMB Express

Table 3 Distribution of the 330 isolates tested, depending on the species, phenotypic test (E-test) and DNA-based test

From: A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae

	E-test	DNA-based test				n
	E-test	CTX-M	TEM	SHV	Interpretation	n
E. cloacae, n = 16	ESBL	Absent	Wild-type	Mutated	ESBL	1
		Present	Absent	Absent	ESBL	1
		Present	Wild-type	Absent	ESBL	8
	ND	Absent	Wild-type	Mutated	ESBL	1
	ND	Present	Wild-type	Absent	ESBL	4
	Non-ESBL	Absent	Wild-type	Mutated	ESBL*	1*
E. coli, n = 119	ESBL	Present	Absent	Absent	ESBL	34
			Wild-type	Absent	ESBL	50
			Wild-type	Mutated	ESBL	2
	ND	Present	Wild-type	Absent	ESBL	20
	Non-ESBL	Absent	Absent	Absent	Non-ESBL	3
	Non-ESBL	Absent	Wild-type	Absent	Non-ESBL	10
K. pneumoniae, n = 147	ESBL	Absent	Absent	Absent	Non-ESBL*	1*
		Absent	Wild-type	Mutated	ESBL	8
		Present	Absent	Wild-type	ESBL	4
			Wild-type	Absent	ESBL	4
				Wild-type	ESBL	73
				Mutated	ESBL	41
	ND	Absent	Absent	Absent	Non-ESBL	2
		Absent	Wild-type	Mutated	ESBL	3
		Present	Wild-type	Absent	ESBL	1
				Wild-type	ESBL	5
				Mutated	ESBL	1
	Non-ESBL	Absent	Absent	Absent	Non-ESBL	2
			Absent	Wild-type	Non-ESBL	1
			Wild-type	Wild-type	Non-ESBL	1
Klebsiella spp., n = 30	ESBL	Present	Absent	Wild-type	ESBL	1
			Wild-type	Absent	ESBL	13
				Wild-type	ESBL	5
				Mutated	ESBL	10
	ND	Present	Wild-type	Absent	ESBL	1
P. mirabilis, n = 10	ESBL	Present	Wild-type	Absent	ESBL	1
				Wild-type	ESBL	1
				Mutated	ESBL	2
	ND	Absent	Wild-type	Mutated	ESBL	3
	ND	Present	Absent	Absent	ESBL	1
	Non-ESBL	Absent	Absent	Absent	Non-ESBL	2
S. marcescens, n = 6	ESBL	Present	Wild-type	Absent	ESBL	5
S. marcescens, n = 6	ND	Present	Wild-type	Absent	ESBL	1
M. morganii, n = 2	ESBL	Present	Absent	Absent	ESBL	1
M. morganii, n = 2	ESBL	Present	Wild-type	Absent	ESBL	1

* Isolates for which genotypic and phenotypic results are discordant. Both discordant isolates and isolates for which E-test yielded a ‘ND’ result (n = 43) were re-tested using the combination disk test (CDT).

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