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AMB Express

Table 3 Distribution of the 330 isolates tested, depending on the species, phenotypic test (E-test) and DNA-based test

From: A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae

  E-test DNA-based test n
CTX-M TEM SHV Interpretation
E. cloacae, n = 16 ESBL Absent Wild-type Mutated ESBL 1
Present Absent Absent ESBL 1
Wild-type Absent ESBL 8
ND Absent Wild-type Mutated ESBL 1
Present Wild-type Absent ESBL 4
Non-ESBL Absent Wild-type Mutated ESBL* 1*
E. coli, n = 119 ESBL Present Absent Absent ESBL 34
Wild-type Absent ESBL 50
Mutated ESBL 2
ND Present Wild-type Absent ESBL 20
Non-ESBL Absent Absent Absent Non-ESBL 3
Absent Wild-type Absent Non-ESBL 10
K. pneumoniae, n = 147 ESBL Absent Absent Absent Non-ESBL* 1*
Wild-type Mutated ESBL 8
Present Absent Wild-type ESBL 4
Wild-type Absent ESBL 4
Wild-type ESBL 73
Mutated ESBL 41
ND Absent Absent Absent Non-ESBL 2
Wild-type Mutated ESBL 3
Present Wild-type Absent ESBL 1
Wild-type ESBL 5
Mutated ESBL 1
Non-ESBL Absent Absent Absent Non-ESBL 2
Wild-type Non-ESBL 1
Wild-type Wild-type Non-ESBL 1
Klebsiella spp., n = 30 ESBL Present Absent Wild-type ESBL 1
Wild-type Absent ESBL 13
Wild-type ESBL 5
Mutated ESBL 10
ND Present Wild-type Absent ESBL 1
P. mirabilis, n = 10 ESBL Present Wild-type Absent ESBL 1
Wild-type ESBL 1
Mutated ESBL 2
ND Absent Wild-type Mutated ESBL 3
Present Absent Absent ESBL 1
Non-ESBL Absent Absent Absent Non-ESBL 2
S. marcescens, n = 6 ESBL Present Wild-type Absent ESBL 5
ND Present Wild-type Absent ESBL 1
M. morganii, n = 2 ESBL Present Absent Absent ESBL 1
Wild-type Absent ESBL 1
  1. * Isolates for which genotypic and phenotypic results are discordant. Both discordant isolates and isolates for which E-test yielded a ‘ND’ result (n = 43) were re-tested using the combination disk test (CDT).
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