|
Target gene
|
Primer name
|
Sequence 5′–3′
|
qPCR
|
Pyrosequencing assay
|
Reference
|
|---|
|
blaTEM
|
TEM-Forward
|
biotin-CGCCGCATACACTATTCTCA
|
Simplex
|
Triplex
|
Own primer design
|
|
TEM-Reverse
|
ATACGGGAGGGCTTACCATC
|
|
TEM-seq104
|
TGCTTTTCTGTGACTGGTG
|
|
TEM-seq164
|
TTCAGCTCCGGTTCC
|
|
TEM-seq238
|
CGCKAGAHCCACGCT
|
|
blaSHV
|
SHV-Forward
|
biotin-TCTGYGCCGCCGTCATTA
|
Simplex
|
Duplex
|
Own primer design
|
|
SHV-Reverse
|
CTTTGTTATTCGGGCCAAGCA
|
|
SVH-seq179
|
GCTGGCCGGGGATGTG
|
|
SVH-seq238
|
ATSCCGCGCSCACCCC
|
|
blaCTX-M
|
CTX-1Forward
|
ATGTGCAGYACCAGTAARGTGA
|
Duplex
|
–
|
Naas et al. (2007)
|
|
CTX-1Reverse
|
TGRGMAATCARYTTRTTCAT
|
|
CTX-2Forward
|
TGGTRAYRTGGMTBAARGG
|
|
CTX-2Reverse
|
TGGGTRAARTARGTSACCAGAA
|
- Degenerate base symbols (IUPAC code): K represents G/T, Y is T/C, S is G/C, R is G/A, M is A/C, H is A/T/C and B is G/T/C.
