Table 2 List of primers used for ESBL-encoding gene detection and pyrosequencing in Enterobacteriaceae
Target gene | Primer name | Sequence 5′–3′ | qPCR | Pyrosequencing assay | Reference |
|---|---|---|---|---|---|
blaTEM | TEM-Forward | biotin-CGCCGCATACACTATTCTCA | Simplex | Triplex | Own primer design |
TEM-Reverse | ATACGGGAGGGCTTACCATC | ||||
TEM-seq104 | TGCTTTTCTGTGACTGGTG | ||||
TEM-seq164 | TTCAGCTCCGGTTCC | ||||
TEM-seq238 | CGCKAGAHCCACGCT | ||||
blaSHV | SHV-Forward | biotin-TCTGYGCCGCCGTCATTA | Simplex | Duplex | Own primer design |
SHV-Reverse | CTTTGTTATTCGGGCCAAGCA | ||||
SVH-seq179 | GCTGGCCGGGGATGTG | ||||
SVH-seq238 | ATSCCGCGCSCACCCC | ||||
blaCTX-M | CTX-1Forward | ATGTGCAGYACCAGTAARGTGA | Duplex | – | Naas et al. (2007) |
CTX-1Reverse | TGRGMAATCARYTTRTTCAT | ||||
CTX-2Forward | TGGTRAYRTGGMTBAARGG | ||||
CTX-2Reverse | TGGGTRAARTARGTSACCAGAA |