Table 1 Primers used genes in Staphylococcus epidermidis
Gene | Primer Sequence (5′–3′) | Annealing temperature | Size of product (bp) |
|---|---|---|---|
16s rRNA | F: CCTATAAGACTGGGATAACTTCGGG R: CTTTGAGTTTCAACCTTGCGGTCG | 58 | 791 |
Ica A | F: ACAGTCGCTACGAAAAGAAA R: GGAAATGCCATAATGACAAC | 56 | 103 |
ica B | F: CTGATCAAGAATTTAAATCACAAA R: AAAGTCCCATAAGCCTGTTT | 56 | 302 |
Ica C | F: TAACTTTAGGCGCATATGTTTT R: TTCCAGTTAGGCTGGTATTG | 56 | 400 |
ica D | Ica D F: ATGGTCAAGCCCAGACAGAG Ica D R: CGTGTTTTCAACATTTAATGCAA | 56 | 198 |
ica R | F: TAATCCCGAATTTTTGTGAA R: AACGCAATAACCTTATTTTCC | 56 | 469 |